Three general approaches have been used to develop comparable FT4 and FT3 immunoassay methods: (i) two-step labeled-hormone; (ii) one-step labeled-analog; and (iii) labeled antibody. Guideline 13.

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This is because total hormone concentrations (TT4 and TT3) are measured at nanomolar levels whereas free hormone concentrations (FT4 and FT3) are measured in the picomole range and to be valid ...

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