What kinds of PCR techniques are you most familiar with? Dr. Bronfin: We perform real-time PCR (qPCR) and reverse transcription (RT)-qPCR for clinical diagnostic and clinical trials.

The real-time RT-PCR technique has been used to detect an amplicon that is amplified during the PCR cycling, in real time.

PCR is also being miniaturized, which would make the technique easier to perform outside of the lab, and cheaper. However, miniaturization is not a new concept and has proven challenging thus far [7].

This technique allows scientists to see the results almost immediately while the process is still ongoing, whereas conventional RT–PCR only provides results at the end of the process. Real time RT–PCR ...

In this workshop, explore the importance of sample quality and polymerase selection for PCR quality and yield.

The rate of detection of Shigella and enteroinvasive Escherichia coli (EIEC) using a PCR technique was compared with the rate detected by standard microbiological methods (bacteriology plus ...

PCR Links.com is a commercial-laden site that has a lot to offer. Covering the topic of PCR ("Well, duh"), the site starts simple, mixes it up, and ends strong. On the simple end, visitors can ...

Researchers have found that the clinical application of BCR::ABL1 digital PCR can reliably quantify stable deep molecular remission of chronic myeloid leukemia (CML), which will help to determine ...

Kary Mullis, Inventor of the PCR Technique, Dies The Nobel laureate was a proponent of LSD, a consultant for O.J. Simpson’s legal defense, and the creator of a company that infused jewelry with ...

The development of the polymerase chain reaction (PCR) has been a major breakthrough in the scientific world. Over time, the technique has evolved beyond the confines of its simple initial design ...

A PCR-RFLP diagnostic technique provided effective identification of the species by a unique pattern with the specific restriction enzyme Xapl (ApoI). Phytopathologia Mediterranea is an international ...